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Toolbox: Water-Sediment Screening Tool (WSST)
Water-Sediment Screening Tool (WSST)
Potential use:
Screening tool to determine biodegradation rates and kinetics of organic chemicals in water-sediment systems.
Novelty and background:
Currently available models for the prediction of the environmental fate of chemicals were developed mainly for organic compounds with simple molecular structures. Furthermore, most of the underlying experimental data were generated in qualitative form (e.g. ready vs. not ready biodegradable) and available data sets are typically related to water-only systems and not to sediments, where sorption, ageing, sequestration and cross coupling may affect bioavailability, transformation and degradation. Hence, there is need for reliable, quantitative biodegradation data for more complex environmental matrices.
A water-sediment screening tool (WSST) was developed based on the OECD Guideline 301C (MITI) to generate biodegradation data. The developed WSST is the first water-sediment test system on screening test level.
Description of tool:
The WSST and experimental procedures were tested and validated using aniline (CAS No. 62-53-3) as training substances. The experiences and the data gained from the experiments with the developed water-sediment test system are reproducible and within the expected range. Thus, the WSST is ready for use for the generation of biodegradation data and kinetics, which can be used for hazard assessment, risk assessment and persistency assessment within the context of the new European Community Regulation on chemicals and their safe use (REACH). Due to the integration of the sediment compartment, the environmental fate of organic compounds can be investigated under screening test conditions in consideration of effects like sorption, ageing and sequestration.
A series of biodegradation experiments were performed for organic chemicals of various chemical classes using the WSST and the MITI test system. The evaluation of the results and the corresponding conclusions are in preparation.
Current state:
ready to use
  1. Junker T, Paatzsch C, Knacker T, (submitted) A water-sediment screening tool for measuring biodegradation of organic chemicals Sci Total Environ
  2. Junker T, Knacker T, (2009).  Report on experimental biodegradation kinetics for selected chemicals and mixtures in water and sediment. (NoMiracle Deliverable D.2.3.17)
  3. Junker T, Knacker T, (2008). Report on investigation of important confounding factors and comparison of results with literature data. (NoMiracle Deliverable D.2.3.14)
  4. Junker T, Kötting N, Knacker T, (2007) Report on experimental biodegradation kinetics for selected test compounds in water and sediment and identification of confounding factors. (NoMiracle Deliverable D.2.3.7)
  5. Junker T, Elste C, Knacker T, Meller M, (2006). Report on Experimental biodegradation kinetics for some model compounds in water and sediment. (NoMiracle Deliverable D.2.3.2)
Responsible scientists:
Thomas Junker, ECT Oekotoxikologie GmbH, D-65439 Flörsheim, Germany,
Related to deliverable:
D.2.3.1, D.2.3.2, D.2.3.7, D.2.3.14, D.2.3.17
Tool box form_ECT.doc