Toolbox - Embryotoxicity test with Danio rerio using...

Title:

Embryotoxicity test with Danio rerio using ‘mixtures’ of chemical and natural stressors

Potential use:

A prolonged DarT to investigate effects of chemicals under varying abiotic stress.

Novelty and background:

Zebrafish (Danio rerio) have been used for several years to determine embryotoxicity of enviromental pollutants. Within the NoMiracle project, a prolonged DarT was developed and adjusted to be conducted at different temperatures which differed from the optimal temperature used normally.

Description of tool:

The tool is ready for use. Zebrafish embryo tests have been used for quite a while, nevertheless, tests using different, suboptimal, temperatures as well as the optimal temperature in combination with chemical stress are new. The tests are conducted at three temperatures (28, 30, 33.5°C) above the optimal temperature (26°C). Exposure times were adjusted according to the faster development at higher temperatures. Results of tests were published in [1, 2]

Current state:

ready to use

References:

Osterauer R, Köhler H-R (2008). Temperature-dependent effects of the pesticides thiacloprid and diazinon on the embryonic development of zebrafish (Danio rerio). Aquat. Toxicol. 86, 485-494
Scheil V, Köhler H-R (2009): Influence of nickel chloride, chlorpyrifos and imidacloprid in combination with different temperatures on the embryogenesis of the zebrafish, Danio rerio. Arch. Environ. Contam. Toxicol. 56, 238-243.

Responsible scientists:

Heinz-R. Köhler, University of Tübingen, heinz-r.koehler@uni-tuebingen.de
Volker Scheil, University of Tübingen, volker.scheil@uni-tuebingen.de

Related to deliverable:

D. 3.2.27

Attachments:

Zebra Fish test for natural stressors and chemicals.doc